Product Description
Size: 20ul / 150ul
The MIDN (18939-1-AP) by Proteintech is a Polyclonal antibody targeting MIDN in WB, ELISA applications with reactivity to human, rat samples
18939-1-AP targets MIDN in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
Tested Applications
Positive WB detected in: PC-12 cells, BxPC-3 cells, rat brain tissue
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Background Information
Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.
Specification
Tested Reactivity: human, rat
Cited Reactivity: human, mouse, pig
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag13492 Product name: Recombinant human MIDN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 127-468 aa of BC094778 Sequence: TQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAAAAARGDPSIASPVSSPCRPVSSAARVPPVPTSPSPASPSPITAGSFRSHAASTTCPEQMDCSPTASSSASPGASTTSTPGASPAPRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTTSCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWSPSRKAGRSDSSSSGGGGSPSEASGLGLDFEDSVWKPEANPDIKSEFVVA Predict reactive species
Full Name: midnolin
Calculated Molecular Weight: 468 aa, 49 kDa
Observed Molecular Weight: 45-50 kDa
GenBank Accession Number: BC094778
Gene Symbol: MIDN
Gene ID (NCBI): 90007
RRID: AB_2878569
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen affinity purification
UNIPROT ID: Q504T8
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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