Proteintech, 19124-1-AP, TRPA1 Polyclonal antibody

Size: 20ul / 150ul TRPA1 Polyclonal Antibody for WB, IHC, ELISA 19124-1-AP targets TRPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: SH-SY5Y cells, SK-N-SH cells, pig colon tissue Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue, rat dorsal root ganglion tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: transient receptor potential cation channel, subfamily A, member 1 Calculated Molecular Weight: 140 kDa Observed Molecular Weight: 120-130 kDa GenBank Accession Number: NM_007332 Gene Symbol: TRPA1 Gene ID (NCBI): 8989 RRID: AB_10642143 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75762 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.