Proteintech, 19125-1-AP, CASR Polyclonal antibody

Size: 20ul / 150ul The CASR (19125-1-AP) by Proteintech is a Polyclonal antibody targeting CASR in WB, ELISA applications with reactivity to human, mouse, rat samples 19125-1-AP targets CASR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, COLO 320 cells, HL-60 cells, Jurkat cells, mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information CASR, also named as GPRC2A, PCAR1, belongs to the G-protein coupled receptor 3 family. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. The activity of CASR is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo). The 60-65 kDa band detected by this antibody might represent a degradation product of CASR (PMID: 15718278). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: calcium-sensing receptor Calculated Molecular Weight: 121 kDa Observed Molecular Weight: 120-140 kDa GenBank Accession Number: NM_000388 Gene Symbol: Calcium Sensing Receptor Gene ID (NCBI): 846 RRID: AB_10646434 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P41180 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.