Proteintech, 19427-1-AP, PIH1D1 Polyclonal antibody

Size: 20ul / 150ul The PIH1D1 (19427-1-AP) by Proteintech is a Polyclonal antibody targeting PIH1D1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 19427-1-AP targets PIH1D1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, human testis tissue, human uterus tissue, mouse eye tissue, HeLa cells, mouse ovary tissue, mouse testis tissue, rat testis tissue Positive IP detected in: HeLa cells Positive IHC detected in: human lung tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13759 Product name: Recombinant human PIH1D1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC001108 Sequence: MANPKLLGLELSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLLVPS Predict reactive species Full Name: PIH1 domain containing 1 Calculated Molecular Weight: 290 aa, 32 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC001108 Gene Symbol: PIH1D1 Gene ID (NCBI): 55011 RRID: AB_10640423 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NWS0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.