Proteintech, 19491-1-AP, ATG14/Barkor (N-terminal) Polyclonal antibody

Size: 20ul / 150ul The ATG14/Barkor (N-terminal) (19491-1-AP) by Proteintech is a Polyclonal antibody targeting ATG14/Barkor (N-terminal) in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, canine, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: KIAA0831 Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 65-68 kDa GenBank Accession Number: NM_014924 Gene Symbol: ATG14 Gene ID (NCBI): 22863 RRID: AB_10642701 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6ZNE5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.