Proteintech, 19507-1-AP, MAST3 Polyclonal antibody

Size: 20ul / 150ul The MAST3 (19507-1-AP) by Proteintech is a Polyclonal antibody targeting MAST3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 19507-1-AP targets MAST3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, human brain tissue, mouse lung tissue, mouse brain tissue, rat brain tissue Positive IP detected in: Jurkat cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: microtubule associated serine/threonine kinase 3 Calculated Molecular Weight: 143 kDa Observed Molecular Weight: 144 kDa GenBank Accession Number: NM_015016 Gene Symbol: MAST3 Gene ID (NCBI): 23031 RRID: AB_10642565 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60307 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.