Proteintech, 19515-1-AP, PRSS27 Polyclonal antibody

Size: 20ul / 150ul The PRSS27 (19515-1-AP) by Proteintech is a Polyclonal antibody targeting PRSS27 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 19515-1-AP targets PRSS27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: SW 1990 cells Positive IP detected in: SW 1990 cells Positive IHC detected in: human pancreas tissue, human oesophagus cancer tissue, mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PRSS27, also named as CAPH2, MPN, belongs to the peptidase S1 family. The trypsin-like serine protease marapsin (Prss27) is a member of the large protease gene cluster at human chromosome 16p13.3. The Prss27 gene is expressed by a variety of human, mouse, and opossum tissues. In humans and mice, it is especially abundant in stratified squamous epithelia of cornea, larynx, esophagus, and cervix and is induced in wounded and psoriatic skin (PMID: 23447538). Prss27 expression was restricted to tissues containing stratified squamous epithelia and was absent or only weakly expressed in all other tissues, including the pancreas. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protease, serine 27 Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 28 kDa GenBank Accession Number: NM_031948 Gene Symbol: PRSS27 Gene ID (NCBI): 83886 RRID: AB_2878585 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BQR3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.