Proteintech, 19527-1-AP, SPANXA2/SPANXA1 Polyclonal antibody

Size: 20ul / 150ul The SPANXA2/SPANXA1 (19527-1-AP) by Proteintech is a Polyclonal antibody targeting SPANXA2/SPANXA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 19527-1-AP targets SPANXA2/SPANXA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: DU 145 cells, HEK-293 cells, PC-3 cells Positive IHC detected in: human skin cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SPANX family, member A2 Calculated Molecular Weight: 11 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: NM_145662 Gene Symbol: SPANXA2/SPANXA1 Gene ID (NCBI): 728712 RRID: AB_2878586 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NS26 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.