Proteintech, 19548-1-AP, MYOF Polyclonal antibody

Size: 20ul / 150ul The MYOF (19548-1-AP) by Proteintech is a Polyclonal antibody targeting MYOF in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 19548-1-AP targets MYOF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, human placenta tissue, HeLa cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myoferlin Calculated Molecular Weight: 235 kDa Observed Molecular Weight: 235-250 kDa GenBank Accession Number: NM_133337 Gene Symbol: MYOF Gene ID (NCBI): 26509 RRID: AB_10642558 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZM1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.