Proteintech, 19649-1-AP, Histone H1.2 Polyclonal antibody

Size: 20ul / 150ul The Histone H1 19649-1-AP targets Histone H1.2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: L02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue Positive IP detected in: HeLa cells Positive IHC detected in: human ovary tumor tissue, human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, camelus bactrianus Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7503 Product name: Recombinant human Histone H1.2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-213 aa of BC002649 Sequence: MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKSAKKTPKKAKKPAAATVTKKVAKSPKKAKVAKPKKAAKSAAKAVKPKAAKPKVVKPKKAAPKKK Predict reactive species Full Name: histone cluster 1, H1c Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 32-33 kDa GenBank Accession Number: BC002649 Gene Symbol: Histone H1.2 Gene ID (NCBI): 3006 RRID: AB_10694432 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16403 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.