Proteintech, 19703-1-AP, ND1 Polyclonal antibody

Size: 20ul / 150ul The ND1 (19703-1-AP) by Proteintech is a Polyclonal antibody targeting ND1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 19703-1-AP targets ND1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse liver tissue, mouse heart tissue, mouse kidney tissue, mouse liver mitochondria Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ND1 belongs to the complex I subunit 1 family. ND1 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: NADH dehydrogenase, subunit 1 (complex I) Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 28-38 kDa GenBank Accession Number: NC_012920 Gene Symbol: ND1 Gene ID (NCBI): 4535 RRID: AB_10637853 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P03886 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.