Proteintech, 19722-1-AP, SPTBN1-Specific Polyclonal antibody

Size: 20ul / 150ul The SPTBN1-Specific (19722-1-AP) by Proteintech is a Polyclonal antibody targeting SPTBN1-Specific in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 19722-1-AP targets SPTBN1-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse kidney tissue, mouse lung tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human liver cancer tissue, human lung tissue, human kidney tissue, human pancreas cancer tissue, human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: spectrin, beta, non-erythrocytic 1 Calculated Molecular Weight: 275 kDa Observed Molecular Weight: 275 kDa GenBank Accession Number: NM_003128 Gene Symbol: SPTBN1 Gene ID (NCBI): 6711 RRID: AB_10638469 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01082 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.