Proteintech, 19782-1-AP, APC Polyclonal antibody

Size: 20ul / 150ul The APC (19782-1-AP) by Proteintech is a Polyclonal antibody targeting APC in IHC, ELISA applications with reactivity to human samples 19782-1-AP targets APC in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human breast cancer tissue, human colon tissue, human colon cancer tissue, human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information APC, also named as DP2.5, belongs to the adenomatous polyposis coli (APC) family. APC is a tumor suppressor that regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division, and associates with other proteins involved in cell attachment and signaling. APC promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. It plays a critical role in several cellular processes. APC regulates beta-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. APC activity is correlated with its phosphorylation state. Defects in APC are a cause of familial adenomatous polyposis (FAP) which includes also Gardner syndrome (GS). Defects in APC are a cause of hereditary desmoid disease (HDD) which also known as familial infiltrative fibromatosis (FIF). Defects in APC are a cause of medulloblastoma (MDB) which is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) which also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: adenomatous polyposis coli Calculated Molecular Weight: 312 kDa GenBank Accession Number: NM_000038 Gene Symbol: APC Gene ID (NCBI): 324 RRID: AB_2878605 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25054 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.