Proteintech, 19828-1-AP, HADH Polyclonal antibody

Size: 20ul / 150ul The HADH (19828-1-AP) by Proteintech is a Polyclonal antibody targeting HADH in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 19828-1-AP targets HADH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, A2780 cells, Jurkat cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat heart tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13928 Product name: Recombinant human HADH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC000306 Sequence: MAFVTRQFMRSVSSSSTASASAKKIIVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENPKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK Predict reactive species Full Name: hydroxyacyl-Coenzyme A dehydrogenase Calculated Molecular Weight: 314 aa, 34 kDa Observed Molecular Weight: 30-34 kDa GenBank Accession Number: BC000306 Gene Symbol: HADH Gene ID (NCBI): 3033 RRID: AB_10667408 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16836 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.