Proteintech, 19835-1-AP, RNF126 Polyclonal antibody

Size: 20ul / 150ul The RNF126 (19835-1-AP) by Proteintech is a Polyclonal antibody targeting RNF126 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 19835-1-AP targets RNF126 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, LNCaP cells, NIH/3T3 cells Positive IP detected in: LNCaP cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13816 Product name: Recombinant human RNF126 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-326 aa of BC001442 Sequence: MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQHLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRHEGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATNPPGLTGVSFSSSSSSSSSSSPSNENATWSPLGRPQPPRPLSNLTL Predict reactive species Full Name: ring finger protein 126 Calculated Molecular Weight: 326 aa, 36 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC001442 Gene Symbol: RNF126 Gene ID (NCBI): 55658 RRID: AB_3085588 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BV68 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.