Proteintech, 19943-1-AP, ERBB4 Polyclonal antibody

Size: 20ul / 150ul The ERBB4 (19943-1-AP) by Proteintech is a Polyclonal antibody targeting ERBB4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 19943-1-AP targets ERBB4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, MCF-7 cells, rat brain tissue Positive IHC detected in: mouse lung tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Calculated Molecular Weight: 147 kDa Observed Molecular Weight: 180 kDa, 147 kDa GenBank Accession Number: NM_001042599 Gene Symbol: ERBB4 Gene ID (NCBI): 2066 RRID: AB_10646486 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15303 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.