Proteintech, 19970-1-AP, MAP3K1 Polyclonal antibody

Size: 20ul / 150ul The MAP3K1 (19970-1-AP) by Proteintech is a Polyclonal antibody targeting MAP3K1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 19970-1-AP targets MAP3K1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse embryo tissue, A431 cells, mouse spleen tissue, A549 cells, NIH/3T3 cells, rat spleen tissue Positive IP detected in: NIH/3T3 cells Positive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase kinase kinase 1 Calculated Molecular Weight: 164 kDa Observed Molecular Weight: 164 kDa GenBank Accession Number: NM_005921 Gene Symbol: MAP3K1 Gene ID (NCBI): 4214 RRID: AB_2878633 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13233 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.