Proteintech, 19977-1-AP, MYBPC3 Polyclonal antibody

Size: 20ul / 150ul The MYBPC3 (19977-1-AP) by Proteintech is a Polyclonal antibody targeting MYBPC3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 19977-1-AP targets MYBPC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, rat heart tissue, human heart tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin binding protein C, cardiac Calculated Molecular Weight: 141 kDa Observed Molecular Weight: 141-150 kDa GenBank Accession Number: NM_000256 Gene Symbol: MYBPC3 Gene ID (NCBI): 4607 RRID: AB_10858232 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14896 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.