Proteintech, 19990-1-AP, Hemoglobin Alpha-Specific Polyclonal antibody

Size: 20ul / 150ul The Hemoglobin Alpha-Specific (19990-1-AP) by Proteintech is a Polyclonal antibody targeting Hemoglobin Alpha-Specific in WB, IHC, ELISA applications with reactivity to human samples 19990-1-AP targets Hemoglobin Alpha-Specific in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue, human heart tissue, human spleen tissue Positive IHC detected in: human colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: hemoglobin, alpha 1 Calculated Molecular Weight: 16 kDa Observed Molecular Weight: 14-16 kDa GenBank Accession Number: NM_000558 Gene Symbol: HBA1 Gene ID (NCBI): 3039 RRID: AB_11182500 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P69905 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.