Proteintech, 20010-1-AP, CYP4F2-Specific Polyclonal antibody

Size: 20ul / 150ul The CYP4F2-Specific (20010-1-AP) by Proteintech is a Polyclonal antibody targeting CYP4F2-Specific in WB, IHC, ELISA applications with reactivity to human samples 20010-1-AP targets CYP4F2-Specific in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HuH-7 cells, L02 cells, SMMC-7721 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytochrome P450, family 4, subfamily F, polypeptide 2 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 50-53 kDa GenBank Accession Number: NM_001082 Gene Symbol: CYP4F2 Gene ID (NCBI): 8529 RRID: AB_3085593 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P78329 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.