Proteintech, 20045-1-AP, LPHN3 Polyclonal antibody

Size: 20ul / 150ul The LPHN3 (20045-1-AP) by Proteintech is a Polyclonal antibody targeting LPHN3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 20045-1-AP targets LPHN3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HepG2 cells, mouse colon tissue, COLO 320 cells, rat brain tissue, human liver tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: latrophilin 3 Calculated Molecular Weight: 162 kDa Observed Molecular Weight: 139 kDa GenBank Accession Number: NM_015236 Gene Symbol: LPHN3 Gene ID (NCBI): 23284 RRID: AB_10665552 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HAR2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.