Proteintech, 20050-1-AP, GPR124 Polyclonal antibody

Size: 20ul / 150ul The GPR124 (20050-1-AP) by Proteintech is a Polyclonal antibody targeting GPR124 in IHC, ELISA applications with reactivity to human, mouse samples 20050-1-AP targets GPR124 in IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse small intestine tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GPR124, also named KIAA1531 and TEM5, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. GPR124 is an Orphan receptor. GPR124 is a Tumor endothelial marker (TEM) that is significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. GPR124 is a member of the adhesion family of G protein-coupled receptors and is localized on the surface of endothelial cells. GPR124 is a seven-pass transmembrane receptor, unlike TEM1, TEM7, and TEM8 which span the membrane once. Proteolytically processed soluble GPR124 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. The antibody recognizes near the C-term of GPR124. The antibody can't recognize the isoform3 of GPR124. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: G protein-coupled receptor 124 Calculated Molecular Weight: 143 kDa GenBank Accession Number: NM_032777 Gene Symbol: GPR124 Gene ID (NCBI): 25960 RRID: AB_3669320 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96PE1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.