Proteintech, 20108-1-AP, FAM96B Polyclonal antibody

Size: 20ul / 150ul The FAM96B (20108-1-AP) by Proteintech is a Polyclonal antibody targeting FAM96B in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 20108-1-AP targets FAM96B in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, Ramos cells, HeLa cells, K-562 cells Positive IP detected in: K-562 cells Positive IHC detected in: mouse kidney tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information FAM96B, also known as MIP18 or CIAO2B (Cytosolic iron-sulfur assembly component 2B), is a component of the cytosolic iron-sulfur protein assembly (CIA) complex involved in iron homeostasis and part of the MMXD complex contributed to proper chromosome segregation (PMID: 36503010). FAM96B may act as a scaffold protein to recruit CKB and the other components of the MMXD complex to associate with the spindle and mitotic apparatus and contributes to chromosome segregation. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13827 Product name: Recombinant human FAM96B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC001733 Sequence: MVGGGGVGGGLLENANPLIYQRSGERPVTAGEEDEQVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQVRVQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHAVNKQLADKERVAAALENTHLLEVVNQCLSARS Predict reactive species Full Name: family with sequence similarity 96, member B Calculated Molecular Weight: 163 aa, 18 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC001733 Gene Symbol: FAM96B Gene ID (NCBI): 51647 RRID: AB_10666848 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y3D0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.