Proteintech, 20112-1-AP, OVCA1 Polyclonal antibody

Size: 20ul / 150ul The OVCA1 (20112-1-AP) by Proteintech is a Polyclonal antibody targeting OVCA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 20112-1-AP targets OVCA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information OVCA1, also named as DPH1, is an evolutionarily conserved gene, indicative of a fundamental biological role in a variety of species. In humans, OVCA1 was initially mapped within the deletion region of 17p13.3 involved in early ovarian cancer (PMID: 8564979). Ovca1-null mice, mainly addressed its tumor suppressive role and potential cooperative role in modifying p53-deficient tumorigenesis (PMID: 14744934). OVCA1 acts as a component of an enzyme complex with DPH2, DPH3 and DPH4 that functionally catalyzes the first step of the diphthamide biosynthesis pathway, to posttranslationally modify a conserved histidine residue (His699 in yeast and His715 in human) of eukaryotic elongation factor 2 (eEF-2). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13910 Product name: Recombinant human OVCA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 87-436 aa of BC003099 Sequence: DILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIPMDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKPLSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEAIATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSARPPSAVACEDCSCRDEKVAPLAP Predict reactive species Full Name: DPH1 homolog (S. cerevisiae) Calculated Molecular Weight: 489 aa, 52 kDa Observed Molecular Weight: 40-60 kDa GenBank Accession Number: BC003099 Gene Symbol: DPH1 Gene ID (NCBI): 1801 RRID: AB_10641038 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZG8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.