Proteintech, 20139-1-AP, MCT1 Polyclonal antibody

Size: 20ul / 150ul The MCT1 (20139-1-AP) by Proteintech is a Polyclonal antibody targeting MCT1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 20139-1-AP targets MCT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, HEK-293 cells, HeLa cells Positive IP detected in: Raji cells Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:800-1:3200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MCT1, the SLC16A1 gene product, is a trans-membrane symporter involved in lactate and pyruvate transportation. It plays an important role in lactic acid transport and H+ clearance in cancer cells. MCT overexpression had been observed in various cancers and may play an important role in tumorigenesis. Two isoforms of MCT1 exist due to the alternative splicing, with predicted MW of 54 kDa and 46 kDa, respectively. While western blot analysis detected MCT1 at an apparent molecular mass of 40-50 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14098 Product name: Recombinant human MCT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 451-500 aa of BC026317 Sequence: EQKANEQKKESKEEETSIDVAGKPNEVTKAAESPDQKDTDGGPKEEESPV Predict reactive species Full Name: solute carrier family 16, member 1 (monocarboxylic acid transporter 1) Calculated Molecular Weight: 500 aa, 54 kDa Observed Molecular Weight: 38-45 kDa GenBank Accession Number: BC026317 Gene Symbol: MCT1 Gene ID (NCBI): 6566 RRID: AB_2878645 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P53985 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.