Proteintech, 20234-1-AP, CSNK2B Polyclonal antibody

Size: 20ul / 150ul The CSNK2B (20234-1-AP) by Proteintech is a Polyclonal antibody targeting CSNK2B in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 20234-1-AP targets CSNK2B in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, A431 cells, HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information CSNK2B, also named as CK2N, G5A nd CK II beta, belongs to the casein kinase 2 subunit beta family. CSNK2B participates in Wnt signaling. It plays a complex role in regulating the basal catalytic activity of the alpha subunit. The antibody is specific to CSNK2B. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: casein kinase 2, beta polypeptide Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: NM_001320 Gene Symbol: CSNK2B Gene ID (NCBI): 1460 RRID: AB_10733246 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P67870 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.