Proteintech, 20276-1-AP, LATS2-Specific Polyclonal antibody

Size: 20ul / 150ul The LATS2-Specific (20276-1-AP) by Proteintech is a Polyclonal antibody targeting LATS2-Specific in IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 20276-1-AP targets LATS2-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: HEK-293 cells Positive IHC detected in: human liver cancer tissue, human colon tissue, mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: LATS, large tumor suppressor, homolog 2 (Drosophila) Calculated Molecular Weight: 120 kDa Observed Molecular Weight: 130-150 kDa GenBank Accession Number: NM_014572 Gene Symbol: LATS2 Gene ID (NCBI): 26524 RRID: AB_10697657 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NRM7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.