Proteintech, 20297-1-AP, P2RY2 Polyclonal antibody

Size: 20ul / 150ul The P2RY2 (20297-1-AP) by Proteintech is a Polyclonal antibody targeting P2RY2 in WB, IHC, ELISA applications with reactivity to human samples 20297-1-AP targets P2RY2 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, MCF-7 cells, PC-3 cells, MDA-MB-231 cells Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information P2RY2, also named P2RU1, P2U1, and P2Y2, belongs to the G-protein coupled receptor 1 family. It is a receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Extracellular ATP then acts on purinergic receptors, including P2RY2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14107 Product name: Recombinant human P2RY2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 268-377 aa of BC012104 Sequence: YYSFRSLDLSCHTLNAINMAYKVTRPLASANSCLDPVLYFLAGQSLVRFARDAKPPTGPSPATPARRRLGLRRSDRTDMQRIEDVLGSSEDSRRTESTPAGSENTKDIRL Predict reactive species Full Name: purinergic receptor P2Y, G-protein coupled, 2 Calculated Molecular Weight: 377 aa, 42 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC012104 Gene Symbol: P2RY2 Gene ID (NCBI): 5029 RRID: AB_3669332 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P41231 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.