Proteintech, 20353-1-AP, SRRT Polyclonal antibody

Size: 20ul / 150ul The SRRT (20353-1-AP) by Proteintech is a Polyclonal antibody targeting SRRT in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 20353-1-AP targets SRRT in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells Positive IP detected in: Jurkat cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14196 Product name: Recombinant human SRRT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 527-876 aa of BC000082 Sequence: LIHTLDDRTQLWASEPGTPPLPTSLPSQNPILKNITDYLIEEVSAEEEELLGSSGGAPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEYPNEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKFVTSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQPPGPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGAGAVRPAVPTGGPPYPHAPYGAGRGNYDAFRGQGGYPGKPRNRMVRGDPRAIVEYRDLDAPDDVDFF Predict reactive species Full Name: serrate RNA effector molecule homolog (Arabidopsis) Calculated Molecular Weight: 876 aa, 101 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC000082 Gene Symbol: SRRT Gene ID (NCBI): 51593 RRID: AB_3085603 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BXP5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.