Proteintech, 20358-1-AP, PRKAR1A Polyclonal antibody

Size: 20ul / 150ul The PRKAR1A (20358-1-AP) by Proteintech is a Polyclonal antibody targeting PRKAR1A in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20358-1-AP targets PRKAR1A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse testis tissue, human stomach cancer tissue, mouse brain tissue, rat salivary gland tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14216 Product name: Recombinant human PRKAR1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC036285 Sequence: MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAI Predict reactive species Full Name: protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) Calculated Molecular Weight: 381 aa, 43 kDa Observed Molecular Weight: 43-45 kDa GenBank Accession Number: BC036285 Gene Symbol: PRKAR1A Gene ID (NCBI): 5573 RRID: AB_10697659 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P10644 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.