Proteintech, 20386-1-AP, CLN3 Polyclonal antibody

Size: 20ul / 150ul The CLN3 (20386-1-AP) by Proteintech is a Polyclonal antibody targeting CLN3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20386-1-AP targets CLN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, MCF-7 cells, SH-SY5Y cells, Raji cells Positive IHC detected in: mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane spanning protein that is involved in microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form homodimeric complex (PMID: 10356317; 17286803). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14224 Product name: Recombinant human CLN3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 288-368 aa of BC002394 Sequence: LVVVYFAEYFINQGLFELLFFWNTSLSHAQQYRWYQMLYQAGVFASRSSLRCCRIRFTWALALLQCLNLVFLLADVWFGFL Predict reactive species Full Name: ceroid-lipofuscinosis, neuronal 3 Calculated Molecular Weight: 438 aa, 48 kDa Observed Molecular Weight: 45-48 kDa GenBank Accession Number: BC002394 Gene Symbol: CLN3 Gene ID (NCBI): 1201 RRID: AB_10694819 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13286 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.