Proteintech, 20549-1-AP, TOP2B Polyclonal antibody

Size: 20ul / 150ul TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA 20549-1-AP targets TOP2B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: topoisomerase (DNA) II beta 180kDa Calculated Molecular Weight: 183 kDa Observed Molecular Weight: 183 kDa GenBank Accession Number: NM_001068 Gene Symbol: TOP2B Gene ID (NCBI): 7155 RRID: AB_10700004 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q02880 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.