Proteintech, 20570-1-AP, PAX7 Polyclonal antibody

Size: 20ul / 150ul The PAX7 (20570-1-AP) by Proteintech is a Polyclonal antibody targeting PAX7 in WB, ELISA applications with reactivity to human samples 20570-1-AP targets PAX7 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human samples. Tested Applications Positive WB detected in: NCCIT cell, NCCIT cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Pax7 is a highly conserved transcription factor involved in early development of the neural crest and gastrulation and is a commonly used marker for satellite cells in muscle tissue. This antibody is specific to PAX7. It will not cross-react with other PAX protein family members.What is the molecular weight of Pax7?57 kDaWhat is the role of Pax7?One of the Paired Box (Pax) transcription factors, which are key in early development and organogenesis in vertebrates, Pax7 binds directly to DNA and is key in neural crest induction (PMID: 26410165). One of the earlier markers in this phase of embryogenesis, Pax7 therefore influences the migration and proliferation of multiple lineages of cells (PMID: 22848431).In adult tissue, Pax7 is expressed by the quiescent resident muscle stem cells, also known as satellite cells, making it a useful marker for this cell type. It is essential for their formation and maintenance in their niche. When activated, these cells can proliferate in the normal maintenance of muscle tissue or respond to injury in repair. The co-expression of Pax7 with MyoD in these activated satellite cells regulates muscle cell proliferation (PMID: 16608873).What proteins does Pax7 interact with?An orthologue of Pax7, another transcription factor of the same family, is Pax3. These two proteins can form a heterodimer, binding to DNA to work together in myogenesis but can also function independently and have overlapping roles (PMID: 16380438).What is the role of Pax7 in disease?Pax7 and Pax 3 have both been identified in tumor tissue from the childhood cancer alveolar rhabdomyosarcoma (ARMS), which affects skeletal muscle (PMID: 23206814). Specification Tested Reactivity: human Cited Reactivity: human, rat, pig, bovine, sheep, goat, duck Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: paired box 7 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: NM_002584 Gene Symbol: PAX7 Gene ID (NCBI): 5081 RRID: AB_2878703 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P23759 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.