Proteintech, 20590-1-AP, AIM2 Polyclonal antibody

Size: 20ul / 150ul The AIM2 (20590-1-AP) by Proteintech is a Polyclonal antibody targeting AIM2 in WB, IHC, IP, ELISA applications with reactivity to human samples 20590-1-AP targets AIM2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, Raji cells, A549 cells, HepG2 cells, MCF-7 cells Positive IP detected in: Raji cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria (PMID:22695634). Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity (PMID:16432157,20566831). It has been reported that AIM2 protein levels are significantly elevated in alveolar macrophages of patients with IPF and bleomycin-treated mice (PMID: 38078661). AIM2 inflammasome was also significantly activated, which was also confirmed in Aβ-treated microglial cells (PMID: 34861878) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14520 Product name: Recombinant human AIM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-343 aa of BC010940 Sequence: MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKK Predict reactive species Full Name: absent in melanoma 2 Calculated Molecular Weight: 343 aa, 39 kDa Observed Molecular Weight: 39-42 kDa GenBank Accession Number: BC010940 Gene Symbol: AIM2 Gene ID (NCBI): 9447 RRID: AB_10694420 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14862 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.