Proteintech, 20645-1-AP, Fibrinogen Alpha Chain Polyclonal antibody

Size: 20ul / 150ul The Fibrinogen Alpha Chain (20645-1-AP) by Proteintech is a Polyclonal antibody targeting Fibrinogen Alpha Chain in WB, IHC, ELISA applications with reactivity to human, mouse samples 20645-1-AP targets Fibrinogen Alpha Chain in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue Positive IHC detected in: human liver cancer tissue, human colon tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9567 Product name: Recombinant human FGA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 20-218 aa of BC020764 Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS Predict reactive species Full Name: fibrinogen alpha chain Calculated Molecular Weight: 866 aa, 95 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC020764 Gene Symbol: Fibrinogen Alpha Chain Gene ID (NCBI): 2243 RRID: AB_2878715 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P02671 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.