Proteintech, 20654-1-AP, SMARCB1 Polyclonal antibody

Size: 20ul / 150ul The SMARCB1 (20654-1-AP) by Proteintech is a Polyclonal antibody targeting SMARCB1 in WB, IP, IHC, ELISA applications with reactivity to human samples 20654-1-AP targets SMARCB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, K-562 cells Positive IP detected in: K-562 cells Positive IHC detected in: human lymphoma tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information SMARCB1, also named as BAF47, INI1 and SNF5L1, belongs to the SNF5 family. It is a core component of the BAF (hSWI/SNF) complex. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. SMARCB1 stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. It is involved in activation of CSF1 promoter. SMARCB1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) which also known as malignant rhabdoid tumor (MRT). Defects in SMARCB1 are a cause of schwannomatosis. The antibody is specific to SMARCB1. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: NM_003073 Gene Symbol: SMARCB1 Gene ID (NCBI): 6598 RRID: AB_10695761 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12824 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.