Proteintech, 20695-1-AP, p300 Polyclonal antibody

Size: 20ul / 150ul The p300 (20695-1-AP) by Proteintech is a Polyclonal antibody targeting p300 in WB, ELISA applications with reactivity to human, mouse samples 20695-1-AP targets p300 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, MCF-7 cells, HeLa cells, Jurkat cells, RAW 264.7 cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: E1A binding protein p300 Calculated Molecular Weight: 264 kDa Observed Molecular Weight: 300 kDa GenBank Accession Number: NM_001429 Gene Symbol: EP300 Gene ID (NCBI): 2033 RRID: AB_3085614 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q09472 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.