Proteintech, 20713-1-AP, GHR Polyclonal antibody

Size: 20ul / 150ul The GHR (20713-1-AP) by Proteintech is a Polyclonal antibody targeting GHR in WB, IHC, IF/ICC, ELISA applications with reactivity to human, pig samples 20713-1-AP targets GHR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: pig liver tissue, pig lung tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GH is a peptide hormone with many pleiotropic actions, including the regulation of metabolism, postnatal growth, cognition, immune, cardiac and renal systems and gut function (PMID: 26059750). GH exerts these actions primarily through alterations in gene expression, initiated by activation of its membrane receptor GHR and the resultant activation of the associated JAK2 and Src family kinases. GH receptor (GHR) is a member of the cytokine receptor family. Mutations in GHR gene have been associated with Laron syndrome, also known as the GH insensitivity syndrome (GHIS), a disorder characterized by short stature. Specification Tested Reactivity: human, pig Cited Reactivity: human, pig, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GH binding protein Calculated Molecular Weight: 72 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: NM_000163 Gene Symbol: GHR Gene ID (NCBI): 2690 RRID: AB_2878726 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P10912 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.