Proteintech, 20718-1-AP, TMC1 Polyclonal antibody

Size: 20ul / 150ul The TMC1 (20718-1-AP) by Proteintech is a Polyclonal antibody targeting TMC1 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 20718-1-AP targets TMC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:300-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Transmembrane channel-like protein 1 (TMC1), is an integral membrane protein with six transmembrane domains and cytoplasmically oriented N- and C- termini (PMID: 20672865). Human TMC1 and mouse Tmc1 mRNA expression were detected in human fetal cochlea and inner-ear tissue of postnatal mouse, respectively. TMC1 is required for cochlear hair-cell function (PMID: 11850618). It is a component of hair cell transduction channels and contributes to permeation properties (PMID: 23871232). Mutations in TMC1 gene can cause progressive postlingual hearing loss and profound prelingual deafness (PMID: 11850618). The antibody is specific to TMC1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: transmembrane channel-like 1 Calculated Molecular Weight: 88 kDa Observed Molecular Weight: 88 kDa GenBank Accession Number: NM_138691 Gene Symbol: TMC1 Gene ID (NCBI): 117531 RRID: AB_10693676 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TDI8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.