Proteintech, 20738-1-AP, CFTR Polyclonal antibody

Size: 20ul / 150ul The CFTR (20738-1-AP) by Proteintech is a Polyclonal antibody targeting CFTR in IHC, FC (Intra), ELISA applications with reactivity to human samples 20738-1-AP targets CFTR in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human lung tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HUVEC cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information What is the human cytogenetic location of the CFTR gene?7q31.2What is the predicted molecular weight of CFTR?168 kDaWhere is CFTR expressed?CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.What is the ion selectivity of CFTR?CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.What is the process of CFTR trafficking?The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.What are the separate domains of CFTR?CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.What regulates CFTR?CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.What is the role of CFTR?CFTR functions as a channel for anions, regulating water and ionic homeostasis.What mutations in CFTR leads to protein degradation?Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.How does CFTR differ from other ATP-binding cassette proteins?Unlike other proteins in the ABC family, CFTR functions as an ion channel.PMIDs: 24513531, 24534272, 22966013, 22709980 Specification Tested Reactivity: human Cited Reactivity: human, mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) Calculated Molecular Weight: 168 kDa GenBank Accession Number: NM_000492 Gene Symbol: CFTR Gene ID (NCBI): 1080 RRID: AB_2722558 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13569 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.