Proteintech, 20790-1-AP, KIFC1 Polyclonal antibody

Size: 20ul / 150ul The KIFC1 (20790-1-AP) by Proteintech is a Polyclonal antibody targeting KIFC1 in WB, IHC, ELISA applications with reactivity to human samples 20790-1-AP targets KIFC1 in WB, IHC, IF, ELISA, Peptide array applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, MCF-7 cells Positive IHC detected in: human bowen disease, human placenta tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14492 Product name: Recombinant human KIFC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 382-725 aa of BC000712 Sequence: GLLLFPSGPGGPSDPPTRLSLSRSDERRGTLSGAPAPPPRHDFSFDRVFPPGSGQDEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFTMEGGPGGDPQLEGLIPRALRHLFSVAQELSGQGWTYSFVASYVEIYNETVRDLLATGTRKGQGGECEIRRAGPGSEELTVTNARYVPVSCEKEVDALLHLARQNRAVARTAQNERSSRSHSVFQLQISGEHSSRGLQCGAPLSLVDLAGSERLDPGLALGPGERERLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLGGSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANRK Predict reactive species Full Name: kinesin family member C1 Calculated Molecular Weight: 673 aa, 74 kDa Observed Molecular Weight: 55-70 kDa GenBank Accession Number: BC000712 Gene Symbol: KIFC1 Gene ID (NCBI): 3833 RRID: AB_10755286 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BW19 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.