Proteintech, 20875-1-AP, TTC19 Polyclonal antibody

Size: 20ul / 150ul The TTC19 (20875-1-AP) by Proteintech is a Polyclonal antibody targeting TTC19 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 20875-1-AP targets TTC19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, HeLa cells, rat heart tissue Positive IHC detected in: human breast cancer tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TTC19 is a subunit of mitochondrial respiratory chain complex III and is proposed to preserve the structural and functional integrity of complex III. Loss-of-function mutations in TTC19 have been found in patients with complex III deficiency associated with heterogeneous neurological syndromes. The precursor TTC19 was detected as a 43 kDa cytoplasmic protein, while the mature TTC19 with the mitochondrial targeting sequence cleaved was observed as 35 kDa by SDS-PAGE. (PMID: 21278747) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag14974 Product name: Recombinant human TTC19 protein Source: e coli. -derived, PKG Tag: GST Domain: 41-383 aa of BC011698 Sequence: EVQVPPSRVAPHGRGPGLLPLLAALAWFSRPAAAEEEEQQGADGAAAEDGADEAEAEIIQLLKRAKLSIMKDEPEEAELILHDALRLAYQTDNKKAITYTYDLMANLAFIRGQLENAEQLFKATMSYLLGGGMKQEDNAIIEISLKLASIYAAQNRQEFAVAGYEFCISTLEEKIEREKELAEDIMSVEEKANTHLLLGMCLDACARYLLFSKQPSQAQRMYEKALQISEEIQGERHPQTIVLMSDLATTLDAQGRFDEAYIYMQRASDLARQINHPELHMVLSNLAAVLMHRERYTQAKEIYQEALKQAKLKKDEISVQHIREELAELSKKSRPLTNSVKL Predict reactive species Full Name: tetratricopeptide repeat domain 19 Calculated Molecular Weight: 501 aa, 56 kDa Observed Molecular Weight: 32-35 kDa GenBank Accession Number: BC011698 Gene Symbol: TTC19 Gene ID (NCBI): 54902 RRID: AB_2878755 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6DKK2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.