Proteintech, 20883-1-AP, EPHB4 Polyclonal antibody

Size: 20ul / 150ul The EPHB4 (20883-1-AP) by Proteintech is a Polyclonal antibody targeting EPHB4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20883-1-AP targets EPHB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, rat testis tissue, HeLa cells, HT-1080 cells, PC-3 cells, DU 145 cells Positive IHC detected in: human lung cancer tissue, human pancreas tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HT-29 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10033 Product name: Recombinant human EPHB4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 639-987 aa of BC052804 Sequence: KKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY Predict reactive species Full Name: EPH receptor B4 Calculated Molecular Weight: 987 aa, 108 kDa Observed Molecular Weight: 110-120 kDa GenBank Accession Number: BC052804 Gene Symbol: EPHB4 Gene ID (NCBI): 2050 RRID: AB_10897158 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P54760 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.