Proteintech, 20973-1-AP, INSC Polyclonal antibody

Size: 20ul / 150ul The INSC (20973-1-AP) by Proteintech is a Polyclonal antibody targeting INSC in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20973-1-AP targets INSC in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, mouse kidney tissue Positive IHC detected in: human kidney tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information INSC may function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. It is involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. INSC may play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. The antibody can recognize isoform1, 2,4,5 of INSC. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: inscuteable homolog (Drosophila) Calculated Molecular Weight: 63 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: NM_001031853 Gene Symbol: INSC Gene ID (NCBI): 387755 RRID: AB_10951111 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q1MX18 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.