Proteintech, 20985-1-AP, DEPDC6/Deptor Polyclonal antibody

Size: 20ul / 150ul The DEPDC6/Deptor (20985-1-AP) by Proteintech is a Polyclonal antibody targeting DEPDC6/Deptor in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells Positive IHC detected in: human liver cancer tissue, human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: DEP domain containing 6 Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: NM_022783 Gene Symbol: DEPDC6 Gene ID (NCBI): 64798 RRID: AB_11182391 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TB45 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.