Proteintech, 21069-1-AP, SIM2 Polyclonal antibody

Size: 20ul / 150ul The SIM2 (21069-1-AP) by Proteintech is a Polyclonal antibody targeting SIM2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 21069-1-AP targets SIM2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: COLO 320 cells, human skeletal muscle tissue, HeLa cells, HepG2 cells, U-251 cells Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15204 Product name: Recombinant human SIM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 342-525 aa of BC110444 Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE Predict reactive species Full Name: single-minded homolog 2 (Drosophila) Calculated Molecular Weight: 667 aa, 73 kDa Observed Molecular Weight: 60-64 kDa GenBank Accession Number: BC110444 Gene Symbol: SIM2 Gene ID (NCBI): 6493 RRID: AB_10732844 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14190 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.