Proteintech, 21084-1-AP, PDZD7 Polyclonal antibody

Size: 20ul / 150ul The PDZD7 (21084-1-AP) by Proteintech is a Polyclonal antibody targeting PDZD7 in WB, IHC, ELISA applications with reactivity to human, mouse samples 21084-1-AP targets PDZD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse ear tissue, mouse retina tissue Positive IHC detected in: mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PDZD7, also known as PDZK7, is essential in organizing the USH2 complex at stereocilia ankle links and blocks inhibition of adenylate cyclase activity mediated by ADGRV1. PDZD7 encodes a PDZ domain-containing scaffold protein that is highly expressed in hair cells of the inner ear. PDZD7 is also expressed in the cilia of photoreceptors (PMID: 33530996). Dysfunction or mutations of PDZD7 result in Usher syndrome type 2 (USH2) and Autosomal recessive non-syndromic hearing loss(ARNSHL) (PMID: 29048736, 20440071). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15318 Product name: Recombinant human PDZD7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-319 aa of BC029054 Sequence: MAQGFAVGFDPLGLGDLSSGSLSSLSSRGHLGSDSGSTATRYLLRKQQRLLNGPPRGIRASSPMGRVILINSPIEANSDESDIIHSVRVEKSPAGRLGFSVRGGSEHGLGIFVSKVEEGSSAERAGLCVGDKITEVNGLSLESTTMGSAVKVLTSSSRLHMMVRRMGRVPGIKFSKEKTTWVDVVNRRLVVEKCGSTPSDTSSEDGVRRIVHLYTTSDDFCLGFNIRGGKEFGLGIYVSKVDHGGLAEENGIKVGDQVLAANGVRFDDISHSQAVEVLKGQTHIMLTIKETGRYPAYKEMVSEYCWLDRLSNGVLQQLS Predict reactive species Full Name: PDZ domain containing 7 Calculated Molecular Weight: 517 aa, 56 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC029054 Gene Symbol: PDZD7 Gene ID (NCBI): 79955 RRID: AB_3085636 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H5P4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.