Proteintech, 21091-1-AP, HDDC3 Polyclonal antibody

Size: 20ul / 150ul The HDDC3 (21091-1-AP) by Proteintech is a Polyclonal antibody targeting HDDC3 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 21091-1-AP targets HDDC3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, human heart tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue, A549 cells, rat heart tissue Positive IP detected in: HeLa cells Positive IHC detected in: human prostate cancer tissue, human brain tissue, human breast cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15342 Product name: Recombinant human HDDC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC033794 Sequence: MGSEAAQLLEAADFAARKHRQQRRKDPEGTPYINHPIGVARILTHEAGITDIVVLQAALLHDTVEDTDTTLDEVELHFGAQVRRLVEEVTDDKTLPKLERKRLQVEQAPHSSPGAKLVKLADKLYNLRDLNRCTPEVKIQ Predict reactive species Full Name: HD domain containing 3 Calculated Molecular Weight: 179 aa, 20 kDa Observed Molecular Weight: 22-25 kDa GenBank Accession Number: BC033794 Gene Symbol: HDDC3 Gene ID (NCBI): 374659 RRID: AB_10733121 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N4P3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.