Proteintech, 21129-1-AP, KIAA1199 / CEMIP Polyclonal antibody

Size: 20ul / 150ul The KIAA1199 / CEMIP (21129-1-AP) by Proteintech is a Polyclonal antibody targeting KIAA1199 / CEMIP in WB, IHC, ELISA applications with reactivity to human, mouse samples 21129-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, COLO 320 cells, U-87 MG cells, HCT 116 cells, SGC-7901 cells, U2OS cells Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, marmoset Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15527 Product name: Recombinant human KIAA1199 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 31-386 aa of BC020256 Sequence: TVAAGCPDQSPELQPWNPGHDQDHHVHIGQGKTLLLTSSATVYSIHISEGGKLVIKDHDEPIVLRTRHILIDNGGELHAGSALCPFQGNFTIILYGRADEGIQPDPYYGLKYIGVGKGGALELHGQKKLSWTFLNKTLHPGGMAEGGYFFERSWGHRGVIVHVIDPKSGTVIHSDRFDTYRSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDMARKAMTKLGSKHFLHLGFRHPWSFLTVKGNPSSSVEDHIEYHGHRGSAAARVFKLFQTEHGEYFNVSLSSEWVQDVEWTEWFDHDKVSQTKGGEKISDLWKAHPGKICNRPIDIQATTMDGVNLSTEVVYKKGQDYRFA Predict reactive species Full Name: KIAA1199 / CEMIP Calculated Molecular Weight: 1361 aa, 153 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC020256 Gene Symbol: KIAA1199 Gene ID (NCBI): 57214 RRID: AB_10695771 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WUJ3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.