Proteintech, 21319-1-AP, GTSE1 Polyclonal antibody

Size: 20ul / 150ul The GTSE1 (21319-1-AP) by Proteintech is a Polyclonal antibody targeting GTSE1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 21319-1-AP targets GTSE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells, MCF-7 cells, mouse liver tissue, K-562 cells, L02 cells, rat liver tissue Positive IHC detected in: human liver cancer tissue, mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13497 Product name: Recombinant human GTSE1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 416-720 aa of BC006325 Sequence: CAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSARRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF Predict reactive species Full Name: G-2 and S-phase expressed 1 Calculated Molecular Weight: 77 kDa Observed Molecular Weight: 66-77 kDa, 100 kDa GenBank Accession Number: BC006325 Gene Symbol: GTSE1 Gene ID (NCBI): 51512 RRID: AB_10733644 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NYZ3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.